Cov ntsiab lus hauv qab no yuav tsum tau sau tseg:
1) Thaum ua qhov kev sim ntawm culturing hlwb, xyuam xim rau kev ua haujlwm aseptic, sterilize cov reagents (kev sib cais, tshuaj ntxuav, thiab lwm yam) thiab cov twj paj nruag.Qhov kev ua haujlwm no yuav tsum tau ua haujlwm los ntawm cov kws tshaj lij los lav qhov kev sib koom ua ke ntawm kev ua haujlwm.
2) Centrifugation kub yog ib txwm nyob rau hauv chav tsev kub (2 ~ 25 ℃).
3) Feem ntau thaum sib cais mononuclear hlwb (PBMC) nrog Ficoll, qhov ntau ntawm cov qe ntshav liab yog me me, uas tuaj yeem cuam tshuam qhov tseeb ntawm qhov kev sim.Yog li lysate tuaj yeem siv tau (qee qhov yuav tsum tau ua kom tsis muaj menyuam), tswj lub sijhawm lysis thiab tiv thaiv kom tsis txhob cuam tshuam cov haujlwm ntawm cov hlwb mononuclear.
4) Ceev faj txog qhov rov centrifugation uas tuaj yeem ua ob npaug rau qhov dilution.