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Kev tsim kho kev lag luam sib txawv thiab cov xov xwm tshiab
Thaum xyoo 1940s, lub tshuab nqus tsev sau cov ntshav tau tsim, uas tshem tawm cov kauj ruam uas tsis tsim nyog xws li kos lub raj thiab thawb cov ntshav mus rau hauv lub raj kuaj, thiab siv lub tshuab nqus tsev tsis siv neeg cov ntshav pub ua ntej tsim hauv lub tshuab nqus tsev kom txo tau cov hemolysis mus rau ib qho. qhov loj.Lwm lub tuam txhab kho mob kuj tau qhia txog lawv tus kheej cov khoom ntim cov ntshav, thiab hauv xyoo 1980s, tau nthuav tawm lub hau npog tshiab rau kev nyab xeeb tube npog.Lub hau kev nyab xeeb muaj ib lub hnab yas tshwj xeeb uas npog lub tshuab nqus tsev vacuum thiab cov roj hmab uas tsim los tshiab.Kev sib xyaw ua ke txo qhov kev pheej hmoo ntawm kev sib cuag nrog cov ntsiab lus ntawm lub raj thiab tiv thaiv cov ntiv tes sib chwv nrog cov ntshav seem nyob rau saum thiab kawg ntawm lub ntsaws.Qhov kev sau lub tshuab nqus tsev no nrog lub hau kev nyab xeeb zoo heev txo qhov kev pheej hmoo ntawm kev kis kab mob los ntawm cov neeg ua haujlwm kho mob los ntawm kev sau mus rau kev ua ntshav.Vim nws huv, nyab xeeb, yooj yim thiab txhim khu kev qha nta istics, cov ntshav sau system tau dav siv nyob rau hauv lub ntiaj teb no thiab tau pom zoo los ntawm NCCLS raws li tus qauv ntsuas rau cov ntshav sau.Lub tshuab nqus tsev nqus tau siv rau hauv qee lub tsev kho mob hauv Suav teb thaum nruab nrab-1990s.Tam sim no, lub tshuab nqus tsev sau cov ntshav tau txais dav hauv ntau lub tsev kho mob hauv nroog loj thiab nruab nrab.Raws li txoj hauv kev tshiab ntawm kev sau cov ntshav thiab kuaj pom, lub tshuab nqus tsev nqus ntshav yog lub kiv puag ncig ntawm kev sau cov ntshav thiab khaws cia.
Phau Ntawv Qhia Kev Ua Haujlwm
Cov txheej txheem sau cov qauv
1. Xaiv cov raj uas tsim nyog thiab koob sau cov ntshav (los yog cov khoom ntim ntshav).
2. Maj mam kais cov raj uas muaj cov tshuaj ntxiv kom tshem tawm cov khoom siv uas yuav ua rau lub stopper.
3. Siv lub tourniquet thiab ntxuav qhov chaw venipuncture nrog cov tshuaj tua kab mob tsim nyog.
4. Nco ntsoov muab tus neeg mob caj npab tso rau hauv qhov chaw qis.
5. Tshem lub hau npog thiab tom qab ntawd ua cov venipuncture.
6. Thaum cov ntshav tshwm, puncture lub roj hmab stopper ntawm lub raj thiab loosen lub tourniquet sai li sai tau.Cov ntshav yuav ntws mus rau hauv lub raj cia li.
7. Thaum thawj lub raj puv tag (cov ntshav tsis ntws mus rau hauv lub raj), maj mam tshem lub raj thiab hloov lub raj tshiab.(Saib rau Daim Ntawv Pom Zoo Ntawm Kev Draw)
8. Thaum lub raj kawg puv, tshem cov koob ntawm cov hlab ntsha.Siv lub tshuab ziab khaub ncaws qhuav los nias qhov chaw puncture kom txog thaum cov ntshav tso tseg.
9. Yog hais tias lub raj muaj cov additive, maj mam tig lub raj 5-8 zaug tam sim ntawd tom qab sau cov ntshav kom paub meej tias cov khoom sib tov txaus thiab cov ntshav.
10. Lub raj tsis muaj tshuaj ntxiv yuav tsum tau centrifuged tsis pub dhau 60-90 feeb tom qab sau cov ntshav.Lub raj muaj cov hlab ntshav yuav tsum tau centrifuged tsis pub dhau 15-30 feeb tom qab sau cov ntshav.Lub centrifugal ceev yuav tsum yog 3500-4500 rpm / min (tus txheeb ze centrifugal quab yuam> 1600gn) rau 6-10 feeb.
11. Kev kuaj ntshav tag nrho yuav tsum tau ua tsis pub dhau 4 teev.Cov qauv ntshav plasma thiab cov khoom kuaj ntshav sib cais yuav tsum tau kuaj yam tsis muaj ncua tom qab sau.Cov qauv yuav tsum muab khaws cia ntawm qhov ntsuas kub yog tias qhov ntsuas tsis tuaj yeem ua raws sijhawm.
Cov ntaub ntawv xav tau tab sis tsis muab
Cov koob txhaj tshuaj thiab cov neeg tuav ntshav (lossis cov khoom ntim ntshav)
Tourniquet
Cawv swab
Pom zoo Order Of Draw
Tube xiav, liab raj, txiv kab ntxwv raj, daj raj, ntsuab raj, lavender raj, dub raj, grey raj
Nco tseg:Nws raug nquahu kom siv lub raj pov tseg (lub raj liab) ua thawj lub raj yuav tsum tau kos yog tias siv cov txheej txheem sau ntshav.Qhov ntawd yuav ua kom ntseeg tau tias cov tshuaj tiv thaiv coagulant-rau-blood ratio.
Lus Ceeb Toom Thiab Kev Ceeb Toom
1. Rau hauv vitro siv nkaus xwb.
2. Tsis txhob siv cov raj tom qab hnub tas sij hawm.
3. Tsis txhob siv cov raj yog tias cov hlab ntsha tawg.
4. Tsuas yog siv ib zaug xwb.
5. Tsis txhob siv cov raj yog tias muaj cov khoom txawv teb chaws.
6. Cov hlab nrog STERILE cim tau ua kom tsis muaj menyuam siv Co60.
7. Cov lus qhia yuav tsum ua raws li qhov tseeb kom ua tau zoo.
8. Lub raj muaj cov hlab ntsha activator yuav tsum tau centrifuged tom qab cov ntshav ua tiav coagulation.
9. Tsis txhob raug cov raj raug tshav ntuj ncaj qha.
10. Hnav cov hnab looj tes thaum lub sij hawm venipuncture kom txo tau qhov raug mob
Cia
Khaws cov hlab ntawm 18-30 ° C, av noo 40-65% thiab tsis txhob raug tshav ntuj ncaj qha.Tsis txhob siv cov raj tom qab lawv hnub tas sij hawm teev tseg rau ntawm daim ntawv lo.