Nucleic Acid Detection Dawb Tube
Lus piav qhia luv luv:
Nws yog tshwj xeeb yog siv rau kev tshawb nrhiav nucleic acid, thiab tau ua tiav raws li kev ua kom huv si, uas txo qis kev kis kab mob thaum lub sijhawm tsim khoom thiab txo cov kev cuam tshuam ntawm kev tuaj yeem nqa mus los ntawm kev sim.
1. Suction ntim sim: Lub nqus ntim, uas yog, cov ntshav nqus tau, muaj qhov yuam kev hauv ± 10%, txwv tsis pub nws tsis tsim nyog.Tus nqi ntawm cov ntshav tsis raug yog qhov teeb meem loj tam sim no.Qhov no tsis yog tsuas yog ua rau kev soj ntsuam tsis raug, tab sis kuj ua rau txhaws thiab puas tsuaj rau cov cuab yeej tshuaj xyuas.
2. Kev sim ua kom lub thawv ntim: Lub tshuab nqus cov ntshav uas muaj cov tshuaj sodium fluorescein composite tau muab tso rau hauv cov dej deionized rau 60 feeb.Nyob rau hauv qhov ntev-yoj ultraviolet lub teeb qhov chaw, tsis muaj fluorescence tau pom nyob rau hauv ib txwm pom kev nyob rau hauv qhov tsaus ntuj nti, uas yog tsim nyog.Cov dej ntws tawm ntawm lub thawv yog qhov laj thawj tseem ceeb rau cov ntshav ntim tsis raug ntawm lub tshuab nqus tsev nqus cov ntshav tam sim no.
3. Kev ntsuas lub zog ntawm lub thawv: lub thawv yog raug rau lub centrifuge nrog lub centrifugal acceleration ntawm 3000g rau 10 feeb, thiab nws yog tsim nyog yog hais tias nws tsis rupture.Cov kev cai nruj me ntsis txawv teb chaws yog: 2 meters saum toj no hauv av, lub tshuab nqus tsev nqus cov hlab ntshav poob vertically yam tsis muaj kev tawg, uas tuaj yeem tiv thaiv kev puas tsuaj rau lub raj kuaj thiab poob ntawm cov qauv.
4. Yam tsawg kawg ntawm qhov chaw pub dawb: Qhov chaw tsawg kawg nkaus kom ntseeg tau tias cov ntshav sib xyaw ua ke.Cov ntshav kos yog 0.5ml-5ml, > + 25% ntawm cov ntshav kos;Yog tias cov ntshav kos yog> 5ml,> 15% ntawm cov ntshav kos.
5. Kev sim ua kom raug ntawm cov kuab tshuaj, qhov sib piv loj thiab cov tshuaj ntxiv ntxiv: qhov yuam kev yuav tsum tsis pub dhau ± 10% ntawm cov qauv cog lus.Qhov no yog ib qho yooj yim overlooked thiab muaj teeb meem, thiab nws yog ib qho ntawm cov laj thawj tseem ceeb rau cov ntaub ntawv xeem tsis raug.
