PRP Tubes Acd Tubes
Lus piav qhia luv luv:
Anticoagulant Citrate Dextrose Solution, feem ntau hu ua ACD-A lossis Tshuaj A yog cov tshuaj tsis muaj pyrogenic, tsis muaj tshuaj lom.Cov khoom no yog siv los ua cov tshuaj tiv thaiv coagulant hauv kev tsim cov platelet-nplua nuj plasma (PRP) nrog PRP Systems rau kev ua cov ntshav extracorporeal.
Anticoagulant Citrate Dextrose Solution, feem ntau hu ua ACD-A lossis Tshuaj A yog cov tshuaj tsis muaj pyrogenic, tsis muaj tshuaj lom.Cov khoom no yog siv los ua cov tshuaj tiv thaiv coagulant hauv kev tsim cov platelet-nplua nuj plasma (PRP) nrog PRP Systems rau kev ua cov ntshav extracorporeal.Anticoagulants uas yog citrate-raws li siv lub peev xwm ntawm citrate ion los chelate ionized calcium uas muaj nyob rau hauv cov ntshav los tiv thaiv cov ntshav coagulation thiab tsim ib qho tsis-ionized calcium-citrate complex.
Tsuas yog cov khoom lag luam anticoagulant uas United States Food & Drug Administration tau pom zoo los siv rau kev npaj PRP hauv ntau PRP Systems yog ACD-A.Raws li kev tshawb fawb tau ua nyob rau xyoo 2016 ntawm PRP tau txais nrog ntau yam tshuaj tiv thaiv coagulants thiab lawv cov teebmeem ntawm tus cwj pwm ntawm mesenchymal stromal hlwb hauv vitro thiab platelet tooj, muaj txiaj ntsig zoo hauv kev siv PRP rau kev kho cov nqaij mos.
Rau kev cais cov platelets nws raug nquahu kom hloov tus qauv sodium citrate rau Acid Citrate Dextrose (ACD-A) raws li cov txheej txheem cais yuav tsum tau ua ntau yam kev ntxuav.Platelets muaj kev ruaj khov dua ntawm 37C thaum lub sij hawm tig, tab sis spinning lawv ntawm chav tsev kub (25 C) kuj ua haujlwm zoo.Txo cov pH los ntawm ACD-A (nws tau nyob ze 6.5) pab kom tsis txhob ua kom cov thrombin seem nyob rau hauv cov hlab ntsha platelet, thiab txhawb nqa tag nrho kev saib xyuas ntawm platelet morphology thaum hloov kev ua haujlwm mus rau qhov tsawg.Nquag koj yuav tsum tau rov muab cov platelets rov ua kom zoo Tyrode Buffer (pH 7.4) thiaj li yuav rov qab tau lawv cov haujlwm.ACD muaj ntau qhov zoo thaum nws los khaws cov platelets
Thaum siv ACD, cov txiaj ntsig tau pom cov txiaj ntsig platelet ntau dua hauv cov ntshav tag nrho.Txawm li cas los xij, kev siv EDTA kuj txhawb kev loj hlob ntawm qhov nruab nrab platelet ntim tom qab cov ntshav centrifugation tau ua tiav kom tau txais PRP.Tom qab ntawd, kev siv ACD ua rau muaj kev loj hlob ntawm mesenchymal stromal hlwb.Yog li ntawd, nws tau txiav txim siab tias cov tshuaj tiv thaiv coagulants suav nrog ACD-A ua lub luag haujlwm tseem ceeb hauv kev npaj PRP thiab pab txhim kho cov txheej txheem kom zoo.
