Platelet-nplua nuj plasma (PRP) yog ib qho autologous concentration ntawm tib neeg platelets hauv plasma.Los ntawm degranulation ntawm alpha granules hauv platelets, PRP tuaj yeem zais ntau yam kev loj hlob, suav nrog platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), hepatocyte growth factor (HGF), thiab transforming. Kev loj hlob zoo tshaj plaws (TGF), uas tau raug sau tseg los pib kho qhov txhab thiab txhawb kev loj hlob thiab hloov pauv ntawm cov hlwb endothelial thiab pericytes rau hauv cov kab mob endothelial.
Lub luag haujlwm ntawm PRP rau kev kho cov plaub hau kev loj hlob tau raug tshaj tawm hauv ntau qhov kev tshawb fawb tsis ntev los no.Uebel et al.tau pom tias platelet plasma kev loj hlob yam tseem ceeb ua rau cov txiaj ntsig ntawm follicular units hauv cov txiv neej qauv baldness phais.Kev ua haujlwm tsis ntev los no tau pom tias PRP ua rau muaj kev loj hlob ntawm dermal papilla hlwb thiab ua rau muaj kev hloov pauv sai ntawm telogen-rau-anagen siv hauv vivo thiab hauv vitro qauv.Lwm txoj kev tshawb fawb tau qhia tias PRP txhawb cov plaub hau follicle reconstitution thiab txo lub sij hawm ntawm cov plaub hau tsim.
Ob leeg PRP thiab platelet-poor plasma (PPP) suav nrog tag nrho cov txiaj ntsig ntawm coagulation proteins.Hauv kev tshawb fawb tam sim no, kev cuam tshuam ntawm PRP thiab PPP ntawm cov plaub hau loj hlob hauv C57BL / 6 nas tau tshawb xyuas.Qhov kev xav yog tias PRP muaj txiaj ntsig zoo rau cov plaub hau ntev thiab nce ntawm cov hauv paus plaub hau.
Kev sim tsiaj
Tag nrho 50 noj qab haus huv C57BL / 6 txiv neej nas (6 lub lis piam, 20 ± 2 g) tau txais los ntawm Center of Laboratory Tsiaj, Hangzhou Normal University (Hangzhou, Suav).Tsiaj txhu tau noj tib yam zaub mov thiab khaws cia rau hauv qhov chaw tas li nyob rau hauv 12: 12-h lub voj voog-teeb.Tom qab 1 lub lis piam ntawm acclimatization, nas tau muab faib ua peb pawg: PRP pawg (n = 10), PPP pawg (n = 10), thiab pawg tswj (n = 10).
Txoj kev tshawb fawb raws tu qauv tau pom zoo los ntawm Pawg Neeg Saib Xyuas Kev Ncaj Ncees ntawm Kev Tshawb Fawb Txog tsiaj raws li Txoj Cai Kev Tshawb Fawb Tsiaj thiab Kev Cai Lij Choj hauv Suav teb.
Kev ntsuas plaub hau ntev
Thaum 8, 13, thiab 18 hnub tom qab txhaj tshuaj kawg, 10 cov plaub hau ntawm txhua tus nas raug xaiv nyob rau hauv lub hom phiaj.Kev ntsuas plaub hau ntev tau ua nyob rau hauv peb qhov chaw siv lub tshuab ntsuas hluav taws xob, thiab lawv qhov nruab nrab tau qhia ua millimeters.Cov plaub hau elongated los yog puas raug tshem tawm.
Hematoxylin thiab eosin (HE) staining
Cov qauv ntawm daim tawv nqaij dorsal raug tshem tawm ntawm 18 hnub tom qab txhaj thib peb.Tom qab ntawd cov qauv raug kho nyob rau hauv 10% nruab nrab buffered formalin, embedded nyob rau hauv paraffin, thiab txiav mus rau hauv 4 μm.Cov seem tau ci rau 4 h rau deparaffinization ntawm 65 ° C, dipped rau hauv gradient ethanol, thiab ces stained nrog hematoxylin rau 5 min.Tom qab sib txawv nyob rau hauv 1% hydrochloric acid cawv, cov seem tau incubated nyob rau hauv ammonia dej, stained nrog eosin, thiab yaug nrog dej distilled.Thaum kawg, cov ntu tau dehydrated nrog gradient ethanol, tshem tawm nrog xylene, mounted nrog nruab nrab resin, thiab soj ntsuam siv lub teeb microscopy (Olympus, Tokyo, Nyiv).
Post lub sij hawm: Oct-12-2022